2-ThioBarbituric Acid Reactive Substances (TBARS) ELISA Kit

Produktnummer ABIN2648832

Dieses Chemical ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Chemical zu quantifizieren.

Kurzübersicht für 2-ThioBarbituric Acid Reactive Substances (TBARS) ELISA Kit (ABIN2648832)

Target: 2-ThioBarbituric Acid Reactive Substances (TBARS)

Reaktivität: Chemical

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma, Urine

Produktdetails

Sensitivität: 0.5 μM

Produktmerkmale: 2-ThioBarbituric Acid Reactive Substances (TBARS) are naturally present in biological specimens and include lipid hydroperoxides and aldehydes which increase in concentration as a response to oxidative stress.1,2 TBARS assay values are usually reported in malonaldehyde (malondialdehyde, MDA) equivalents, a compound that results from the decomposition of polyunsaturated fatty acid lipid peroxides. The TBARS assay is a well-recognized, established method for quantifying these lipid peroxides, although it has been criticized for its reactivity towards other compounds other than MDA.3 This TBARS Cuvette Assay Kit offers the researcher a straightforward, reproducible and consistent method for analyzing urine for lipid peroxidation products.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 0.5 h

Plattentyp: Pre-coated

Protokoll: This TBARS Cuvette Assay Kit is based on the reaction of a chromogenic reagent, 2-thiobarbituric acid, with MDA at 25 °C. One molecule of MDA reacts with 2 Molecules of 2-thiobarbituric acid via a Knoevenagel-type condensation to yield a chromophore with absorbance maximum at 532 nm,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Antigendetails

Target: 2-ThioBarbituric Acid Reactive Substances (TBARS)